A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERS-CoV specific IgG in humans and animals
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منابع مشابه
Fabrication of a Voltammetric Biosensor in Combination with Enzyme-linked Immunosorbent Assay to Detect Aflatoxin M1 in Milks
Background and Objectives: Aflatoxin M1 is one the most important mycotoxin contaminants of milks. Various methods have been described to detect aflatoxins in milks, including chromatography, enzyme-linked immunosorbent assay and colorimetry. Regarding high sensitivity of electrochemical sensor based methods, the aim of the present study was to fabricate an accurate sensitive voltametric biosen...
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An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...
متن کاملenzyme-linked immunosorbent assay
An enzyme-linked immunosorbent assay was used to titrate antisera raised against live cultures of eight type species (biotypes) of Bacteroides with the EDTA-released outer-membrane complex from 29 characterized strains of Bacteroides species. With only minor exceptions, the strains investigated reacted to titre with the antisera raised against the homologous type species and not against the het...
متن کاملApplication of a modified human enzyme-linked immunosorbent assay kit for diagnosis of hydatidosis in sheep
Cystic hydatid disease (hydatidosis) is one of the most important zoonosis that is caused by the larval stage of Echinococcus granulosus . As its diagnosis by clinical symptoms alone is difficult and confusing, serologic diagnostic techniques are used to confirm the disease. These techniques can also be used for epidemiologic studies. The present study was performed with a commercial human en...
متن کاملEnzyme-linked immunosorbent choriomammotropin assay.
We have devloped an enzyme-linked immunosorbent assay for determining choriomammotropin (human placental lactogen) in serum. Unlabeled hormone competes with choriomammotropin-beta-galactosidase conjugate for antibody bound to polystyrene tubes. The entire assay can be performed in 2.5 h with good precision. The coefficient of variation for one sample with a mean concentration of 5.6 mg/L, assay...
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ژورنال
عنوان ژورنال: Biosafety and Health
سال: 2019
ISSN: 2590-0536
DOI: 10.1016/j.bsheal.2019.12.006